SARS-CoV-2 Research Panel

Single day. Single tube. Exceptional data. 

NGS-based SARS-CoV-2 surveillance demands sample prep that is robust to strain variants and easy to implement at scale.

The Molecular Loop SARS-CoV-2 Research Panel is just that.

  • Robust genome capture is enabled by tiled probes that create highly-redundant overlapping amplicons across the entire genome
  • Panel offers comprehensive coverage of the SARS-CoV-2 genome
  • Workflow consists of 4 simple steps following RNA extraction, all in a single well
  • Libraries include UMIs and UDIs to minimize the impact of index hopping

Elegant 4-step workflow with colored master mixes makes implementation simple and easy to scale

RT + Probe Anneal

1-step reverse transcription + annealing of capture probes


Enzymatic circularization of probes to capture viral genome and add UMIs


PCR to add unique dual sample indices and form sequencing library

Pool + Cleanup

Equi-volume pooling of samples and purification prior to sequencing

Comprehensive SARS-CoV-2 genome coverage

The panel provides comprehensive coverage of the SARS-CoV-2 genome with 125K read pairs at 10,000 and 1,000 RNA input copies

For more information, download our SARS-CoV-2 2021 AGBT poster.

Molecular Loop SARS-CoV-2 Sequencing depth
SARS-COV-2 Sequencing Depth. Fig.B

Data shown was generated on an Illumina sequencer. Contact us for information on our PacBio panel. 

Ensuring comprehensive coverage of new variants

Robust Omicron Variant Detection

Nearly 1,000 probes are tiled across the SARS-CoV-2 genome such that they overlap, but each have a unique binding site. As new variants rapidly emerge, this probe redundancy protects the assay from dropouts. Because of this feature, the first Molecular Loop assay developed against the native SARS-CoV-2 strain has proven to have the robustness to ensure comprehensive coverage of all variants that have emerged since 2019.

Molecular Loop tiling

Intended use

The SARS-CoV-2 NGS research panel is a nucleic acid capture-based research-use only assay intended for use in characterization and environmental monitoring of the SARS-CoV-2 virus. This product is for research use only. It is not intended for the diagnosis, prevention, or treatment of any disease or condition.

Molecular Loop Biosciences, Inc. assumes no liability regarding use of the product in applications for which it is not intended.

fraction of SARS-CoV-2 genome coverage at 5x depth with Molecular Loop technology
fraction of SARS-CoV-2 genome coverage at 1x depth with Molecular Loop technology