Contact us for information on our 4-step SARS-CoV-2 research sequencing panel

or download our AGBT 2021 poster note

SARS-CoV-2
Research Panel

Single day. Single tube. Exceptional data. 

NGS-based SARS-CoV-2 surveillance demands sample prep that is robust to strain variants and easy to implement at scale.

The Molecular Loop SARS-CoV-2 Research Panel is just that.

  • Robust genome capture is enabled by tiled probes that create highly-redundant overlapping amplicons across the entire genome
  • Panel offers comprehensive coverage of the SARS-CoV-2 genome
  • Workflow consists of 4 simple steps following RNA extraction, all in a single well
  • Libraries include UMIs to enable deduplication and UDIs to minimize the impact of index hopping

Elegant 4-step workflow with color-coded master mixes makes implementation simple and easy to scale

RT + Probe Anneal

1-step reverse transcription + annealing of capture probes

Circularize

Enzymatic circularization of probes to capture viral genome and add UMIs

PCR

PCR to add unique dual sample indices and form sequencing library

Pool + Cleanup

Equi-volume pooling of samples and purification prior to sequencing

Comprehensive SARS-CoV-2 genome coverage

The panel provides comprehensive coverage of the SARS-CoV-2 genome across 250K and 2.5M deduplicated reads, at real-world levels of RNA input.

For more information, download our 2021 AGBT poster.

SARS-CoV-2 variant detection

Deduplicated coverage depth at sites of known mutations of interest within 3 novel strains

Strain & Region First Detected Spike Protein Mutation Deduplicated Depth of Coverage
16hr Hybridization 4hr Hybridization
2.5M Reads 250K Reads 2.5M Reads 250K Reads
B.1.1.7 United Kingdom 69/70 deletion 251 162 263 182
144Y deletion 172 94 115 82
N501Y 353 200 253 178
A570D 114 72 76 53
D614G 351 219 210 139
P681H 145 103 127 94
P.1 Japan/Brazil E484K 239 141 155 104
K417T 23 9 23 15
N501Y 353 200 253 178
D614G 351 219 210 139
B.1.351 South Africa K417N 21 8 21 14
E484K 239 141 155 104
N501Y 353 200 253 178
D614G 351 219 210 139
Strain & Region First Detected Strain & Region First Detected Deduplicated Depth of Coverage
16hr Hybridization 4hr Hybridization
2.5M Reads 250K Reads 2.5M Reads 250K Reads
B.1.1.7 United Kingdom 69/70 deletion 251 162 263 182
144Y deletion 172 94 115 82
N501Y 353 200 253 178
A570D 114 72 76 53
D614G 351 219 210 139
P681H 145 103 127 94
P.1 Japan/Brazil E484K 239 141 155 104
K417T 23 9 23 15
N501Y 353 200 253 178
D614G 351 219 210 139
B.1.351 South Africa K417N 21 8 21 14
E484K 239 141 155 104
N501Y 353 200 253 178
D614G 351 219 210 139

Intended use

The SARS-CoV-2 NGS research panel is a nucleic acid capture-based research-use only assay intended for use in characterization and environmental monitoring of the SARS-CoV-2 virus. This product is for research use only. It is not intended for the diagnosis, prevention, or treatment of any disease or condition.

Molecular Loop Biosciences, Inc. assumes no liability regarding use of the product in applications for which it is not intended.

fraction of SARS-CoV-2 genome coverage at 1x depth with Molecular Loop technology
fraction of SARS-CoV-2 genome coverage at 5x depth with Molecular Loop technology