SARS-CoV-2 Research Panel
Single day. Single tube. Exceptional data.
NGS-based SARS-CoV-2 surveillance demands sample prep that is robust to strain variants and easy to implement at scale.
The Molecular Loop SARS-CoV-2 Research Panel is just that.
- Robust genome capture is enabled by tiled probes that create highly-redundant overlapping amplicons across the entire genome
- Panel offers comprehensive coverage of the SARS-CoV-2 genome
- Workflow consists of 4 simple steps following RNA extraction, all in a single well
- Libraries include UMIs and UDIs to minimize the impact of index hopping
Elegant 4-step workflow with colored master mixes makes implementation simple and easy to scale
RT + Probe Anneal
1-step reverse transcription + annealing of capture probes
Circularize
Enzymatic circularization of probes to capture viral genome and add UMIs
PCR
PCR to add unique dual sample indices and form sequencing library
Pool + Cleanup
Equi-volume pooling of samples and purification prior to sequencing
Comprehensive SARS-CoV-2 genome coverage
The panel provides comprehensive coverage of the SARS-CoV-2 genome with 125K read pairs at 10,000 and 1,000 RNA input copies
For more information, download our SARS-CoV-2 2021 AGBT poster.
Data shown was generated on an Illumina sequencer. Contact us for information on our PacBio panel.
Ensuring comprehensive coverage of new variants
Robust Omicron Variant Detection
Nearly 1,000 probes are tiled across the SARS-CoV-2 genome such that they overlap, but each have a unique binding site. As new variants rapidly emerge, this probe redundancy protects the assay from dropouts. Because of this feature, the first Molecular Loop assay developed against the native SARS-CoV-2 strain has proven to have the robustness to ensure comprehensive coverage of all variants that have emerged since 2019.
Intended use
The SARS-CoV-2 NGS research panel is a nucleic acid capture-based research-use only assay intended for use in characterization and environmental monitoring of the SARS-CoV-2 virus. This product is for research use only. It is not intended for the diagnosis, prevention, or treatment of any disease or condition.
Molecular Loop Biosciences, Inc. assumes no liability regarding use of the product in applications for which it is not intended.
