SARS-CoV-2
Research Panel

Single day. Single tube. Exceptional data.

NGS-based SARS-CoV-2 surveillance demands sample prep that is robust to strain variants and easy to implement at scale.

The Molecular Loop SARS-CoV-2 Research Panel is just that.

Robust genome capture is enabled by tiled probes that create highly-redundant overlapping amplicons across the entire genome
Panel offers comprehensive coverage of the SARS-CoV-2 genome
Workflow consists of 4 simple steps following RNA extraction, all in a single well
Libraries include UMIs to enable deduplication and UDIs to minimize the impact of index hopping

Elegant 4-step workflow with color-coded master mixes makes implementation simple and easy to scale

RT + Probe Anneal

1-step reverse transcription + annealing of capture probes

Circularize

Enzymatic circularization of probes to capture viral genome and add UMIs

PCR

PCR to add unique dual sample indices and form sequencing library

Pool + Cleanup

Equi-volume pooling of samples and purification prior to sequencing

Comprehensive SARS-CoV-2 genome coverage

The panel provides comprehensive coverage of the SARS-CoV-2 genome across 250K and 2.5M deduplicated reads, at real-world levels of RNA input.

For more information, download our 2021 AGBT poster.

SARS-CoV-2 variant detection

Deduplicated coverage depth at sites of known mutations of interest within 3 novel strains

Strain & Region First Detected	Spike Protein Mutation	Deduplicated Depth of Coverage
		16hr Hybridization		4hr Hybridization
		2.5M Reads	250K Reads	2.5M Reads	250K Reads
B.1.1.7 United Kingdom	69/70 deletion	251	162	263	182
	144Y deletion	172	94	115	82
	N501Y	353	200	253	178
	A570D	114	72	76	53
	D614G	351	219	210	139
	P681H	145	103	127	94
P.1 Japan/Brazil	E484K	239	141	155	104
	K417T	23	9	23	15
	N501Y	353	200	253	178
	D614G	351	219	210	139
B.1.351 South Africa	K417N	21	8	21	14
	E484K	239	141	155	104
	N501Y	353	200	253	178
	D614G	351	219	210	139

Strain & Region First Detected	Strain & Region First Detected	Deduplicated Depth of Coverage
		16hr Hybridization		4hr Hybridization
		2.5M Reads	250K Reads	2.5M Reads	250K Reads
B.1.1.7 United Kingdom	69/70 deletion	251	162	263	182
	144Y deletion	172	94	115	82
	N501Y	353	200	253	178
	A570D	114	72	76	53
	D614G	351	219	210	139
	P681H	145	103	127	94
P.1 Japan/Brazil	E484K	239	141	155	104
	K417T	23	9	23	15
	N501Y	353	200	253	178
	D614G	351	219	210	139
B.1.351 South Africa	K417N	21	8	21	14
	E484K	239	141	155	104
	N501Y	353	200	253	178
	D614G	351	219	210	139

Intended use

The SARS-CoV-2 NGS research panel is a nucleic acid capture-based research-use only assay intended for use in characterization and environmental monitoring of the SARS-CoV-2 virus. This product is for research use only. It is not intended for the diagnosis, prevention, or treatment of any disease or condition.

Molecular Loop Biosciences, Inc. assumes no liability regarding use of the product in applications for which it is not intended.