Simple,
yet refined

Combining powerful science with an easy-to-use workflow for unparalleled production-scale targeted sequencing.

Hybrid capture or amplicons?

Finally, you don’t have to compromise.

Hybrid capture library prep kits provide rich data quality, but complicated and lengthy protocols make this technology difficult to implement in high throughput laboratories.  On the other hand, amplicon-based enrichment kits provide easier workflows and faster turnaround times, but suffer from poor coverage that can result in allelic dropouts. 

Molecular Loop’s chemistry is the result of a decades-long refinement of the molecular inversion probe (MIP) approach that delivers better performance with a simpler workflow to provide the perfect balance of benefits of both hybrid capture- or amplicon-based target enrichment. 

Molecular Loop Capture

Molecular Loop’s chemistry comprises only 4 steps, unlike other enrichment chemistries. Molecular inversion probes (MIPs) are designed to target regions of interest in the sample DNA. The MIPs are hybridized to sample DNA and circularized enzymatically, and then unique molecular identifiers (UMIs) are introduced. Unique dual-index adapters are then added to each DNA fragment by PCR to create the sequencer-ready library. These genetic elements enable sample multiplexing and deduplication for accurate variant calling. In the final step, the amplicons are pooled and purified prior to sequencing. 

Highlights

  • Workflow simpler than either hybrid capture or amplicon-based enrichment
  • Accuracy higher than amplicon-based approaches
  • UMIs and dual indices (unique pairs, not combinatorial) are standard
 

There’s nothing more important than data quality

Molecular Loop provides high coverage, high uniformity, and high confidence results

Accurate data from overlapped tiled amplicons

Each region is covered by multiple independent, non-overlapping priming sites. Strand-specific probes provide complete bidirectional coverage, while UMIs enable error correction & precise quantitation of allelic frequency and genomic provenance.

High uniformity, even in difficult regions

high uniformity

Due to high degrees of secondary structure, 5’ and 3’ untranslated regions (UTRs) can be problematic for some sequencing platforms. Molecular Loop provides high coverage in difficult-to-sequence genomic regions, even those with high secondary structure or homopolymeric regions.

50-100x coverage,
consistently

high coverage

Our panels offer 100x coverage across >99% of bases, even when using our shortened, 4-hour hybridization protocol.  High-confidence results start with high coverage – and we have you covered.

Molecular Loop provides accurate data from overlapped tiled amplicons

Accurate data from overlapped tiled amplicons

Each region is covered by multiple independent, non-overlapping priming sites. Strand-specific probes provide complete bidirectional coverage, while UMIs enable error correction & precise quantitation of allelic frequency and genomic provenance.

SEE THE DATA
Molecular Loop high uniformity

High uniformity, even in difficult regions

Due to high degrees of secondary structure, 5’ and 3’ untranslated regions (UTRs) can be problematic for some sequencing platforms. Molecular Loop provides high coverage in difficult-to-sequence genomic regions, even those with high secondary structure or homopolymeric regions.

SEE THE DATA
Molecular Loop high coverage

50-100x coverage, consistently

Our panels offer 100x coverage across >99% of bases, even when using our shortened, 4-hour hybridization protocol.  High-confidence results start with high coverage – and we have you covered.

SEE THE DATA

Accurate data from overlapped tiled amplicons

Molecular Loop provides accurate data from overlapped tiled amplicons

Each region is covered by multiple independent, non-overlapping priming sites. Strand-specific probes provide complete bidirectional coverage, while UMIs enable error correction & precise quantitation of allelic frequency and genomic provenance.

High uniformity, even in difficult regions

Molecular Loop high uniformity

Due to high degrees of secondary structure, 5’ and 3’ untranslated regions (UTRs) can be problematic for some sequencing platforms. Molecular Loop provides high coverage in difficult-to-sequence genomic regions, even those with high secondary structure or homopolymeric regions.

See the data

50-100x coverage, consistently

Molecular Loop high coverage

Our panels offer 100x coverage across >99% of bases, even when using our shortened, 4-hour hybridization protocol.  High-confidence results start with high coverage – and we have you covered.

See the data

Comparison of library prep protocols

Molecular Loop Hybridization Enrichment Amplicon Enrichment

Chemistry

Molecular Inversion Probe capture

Hybrid capture

Amplicon

Multiplexing

Multiplexing
?
?

Sample input

Multiplexing
Normalization recommended
Normalization recommended

Coverage 

• >99% of target bases

• 5X average redundancy*

?
?

Number of Steps

Multiplexing

14

9

Hands-On time

75 minutes

?
?

Total Time

8 hours

?
?

* 3X average redundancy for low-MW input or cell-free DNA